Journal: Frontiers in Neuroscience

Article Title: Fourier transform infrared spectroscopy detects distinct TAR DNA-binding protein 43 signatures in frontotemporal lobar degeneration

doi: 10.3389/fnins.2025.1649433

Figure Lengend Snippet: Neuropathological findings across study subjects. (A) Staining with AT8 (Tau) across superior and middle temporal samples from each study subject (left panel). (B) Higher magnification AT8 staining (right panel) from representative grey and white matter regions, indicated by squares in the left panel. (C) Immunohistochemical staining with TDP-43 across the preparations from all study subjects (left panel). (D) Corresponding magnification of grey and white matter regions (right panel), indicated by squares in the left panel. (E) Additional magnification and morphological features of TDP-43 aggregates in GM regions from FTLD subjects. Additional whole-section and high magnification images of grey and white matter stained for β-amyloid (F) and proteolipid protein (PLP) (G) are presented for each frontotemporal lobar dementia case. FTLD, frontotemporal lobar dementia; H-AD, high likelihood Alzheimer’s disease; AT8, phosphorylated tau protein; He, hematoxylin; GM, grey matter; WM, white matter; TDP-43, TAR DNA-binding protein 43; PLP, proteolipid protein. NIIs, intranuclear inclusion; NCIs, neuronal cytoplasmic inclusions; DNs, dystrophic neurites.

Article Snippet: Myelin proteolipid protein (PLP) staining (Serotec, Oxford, United Kingdom, 1:500) was performed by standard DAB staining procedures as previously described ( ).

Techniques: Staining, Immunohistochemical staining, Binding Assay